Sequences from confirmation amplicon


Confirmation sequences for line SALK_141625c and hit At3g44110 are shown.
Line ID SALK_141625c
Locus At3g44110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:15869322
Primers used for PCR 30md - LBb3, show primer details
   
Sequence Name 18-K114334-SALK_141625C-30md
GenBank Accession LN484785 [GenBank]
Sequencing primer gene specific primer, 30md: 5'-CAGAAGATCACATTTGAAGGACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K114334-SALK_141625C-30md
TCCGCTTTGATACTAGTATGTTTACTTTTCTTCTGGAGACTAACCTTTTGTTTTATGTTT
TTGTGTTGGTGCAGCCTGACACTGTCACTGGAGATATAGTGTTTGTCCTTCAGCAGAAAG
AGCACCCAAAGTTCAAGAGAAAGGGAGAAGACCTCTTTGTGGAGCACACACTTTCTCTAA
CCGAAGCTTTGTGTGGCTTCCAATTTGTTCTGACTCACTTGGATGGCAGAAGTCTTCTCA
TTAAATCTAATCCTGGGGAGGTCGTGAAACCTGGTACGTATTCAGATGCATCGTCATACT
CAGATTTTACAGTTACTATAACTGTCTCTGATTGAAACATGATCACTTATCTNATGTGTT
TGACAGATTCATACAAGGCAATAAGCGATGAAGGAATGCCGATATACCAGAGGCCATTCA
TGAAGGGTAAGCTCTACATCCACTTCACAGTGGAGTTCCCGGACTCGTTGAGCCCAGATC
AGACCAAACAAATTGTGGTGTAAACAAATTGACGCTNAGACAACTTAATAACACATTGCG
GACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGAT
TGCCCTTCACCGCCNGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTNTGCCCCA
GCAGGCGAANATCCTGTTTGATGAAAGTTC
   
Sequence Name 08-K114333-SALK_141625C-R210
GenBank Accession LN484497 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K114333-SALK_141625C-R210
CCACAATTTGTTTGGTCTGATCTGGGCTCAACGAGTCCGGGAACTCCACTGTGAAGTGGA
TGTAGAGCTTACCCTTCATGAATGGCCTCTGGTATATCGGCATTCCTTCATCGCTTATTG
CCTTGTATGAATCTGTCAAACACATAAGATAAGTGATCATGTTTCAATCAGAGACAGTTA
TAGTAACTGTAAAATCTGAGTATGACGATGCATCTGAATACGTACCAGGTTTCACGACCT
CCCCAGGATTAGATTTAATGAGAAGACTTCTGCCATCCAAGTGAGTCAGAACAAATTGGA
AGCCACACAAAGCTTCGGTTAGAGAAAGTGTGTGCTCCACAAAGAGGTCTTCTCCCTTTC
TCTTGAACTTTGGGTGCTCTTTCTGCTGAAGGACAAACACTATATCTCCNGTGACAGTGT
CAGGCTGCACCAACACAAAAACATAAAACAAAAGGTTAGTCTCCAGAAGAAAAGTAAACA
TACTAGTAATCAAAGCTGGAGTAAATGTATGAGAGTAGATACCGCTTCATCTGCTTGTCC
TTCAATGTGNATCTTCTG

Last Updated on 06.12.2018 16:11