Genotyping and primer details


Genotyping and primer details for SALK_141699c - At1g21190
Line ID SALK_141699c
Locus At1g21190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7420441 of line SALK_141699c Chr1:7420441
   
Insertion allele amplicon LBb3 - 42cv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 42cv: 5'-GATTCGGTTGAGTATCGAAGAGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7420812
   
wt amplicon (over InsPos) 35dz - 42cv
wt amplicon start Chr1:7420012
wt amplicon end Chr1:7420812
wt amplicon size 801 bp
Locus specific primer 42cv: 5'-GATTCGGTTGAGTATCGAAGAGAG-3'
2nd locus specific primer 35dz: 5'-ACACCAATATCACTCCATCTCCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11