Genotyping and primer details


Genotyping and primer details for SALK_142221c - At3g05180
Line ID SALK_142221c
Locus At3g05180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1468931 of line SALK_142221c Chr3:1468931
   
Insertion allele amplicon LBb3 - 64qz
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 64qz: 5'-GGGGGAAATATTTGAATTTGATTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1469340
   
wt amplicon (over InsPos) 31rz - 64qz
wt amplicon start Chr3:1468346
wt amplicon end Chr3:1469340
wt amplicon size 995 bp
Locus specific primer 64qz: 5'-GGGGGAAATATTTGAATTTGATTT-3'
2nd locus specific primer 31rz: 5'-TTTGGTTTGTGGAAAAGTGTTGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11