Genotyping and primer details


Genotyping and primer details for SALK_142273 - At1g69030
Line ID SALK_142273
Locus At1g69030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:25948203 of line SALK_142273 Chr1:25948203
   
Insertion allele amplicon LBb3 - 48wj
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 48wj: 5'-TGCTCAGACGATGCAGTAGATATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:25947770
   
wt amplicon (over InsPos) 06wy - 48wj
wt amplicon start Chr1:25947770
wt amplicon end Chr1:25948764
wt amplicon size 995 bp
Locus specific primer 48wj: 5'-TGCTCAGACGATGCAGTAGATATT-3'
2nd locus specific primer 06wy: 5'-GGAATTGAGGATTTGAAATGTGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11