Genotyping and primer details


Genotyping and primer details for SALK_142359c - At1g01440
Line ID SALK_142359c
Locus At1g01440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:162084 of line SALK_142359c Chr1:162084
   
Insertion allele amplicon LBb3 - 41ea
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 41ea: 5'-TCTTAGGCACATATAGCAGCTCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:161337
   
wt amplicon (over InsPos) 04fo - 41ea
wt amplicon start Chr1:161337
wt amplicon end Chr1:162572
wt amplicon size 1236 bp
Locus specific primer 41ea: 5'-TCTTAGGCACATATAGCAGCTCAA-3'
2nd locus specific primer 04fo: 5'-AGACTAAAAGCTTGATTCTCACCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11