Genotyping and primer details


Genotyping and primer details for SALK_142747c - At1g50890
Line ID SALK_142747c
Locus At1g50890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:18864076 of line SALK_142747c Chr1:18864076
   
Insertion allele amplicon LBb3 - 65cs
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 65cs: 5'-GGCATGTCGTTTTGATAAGGTACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18864636
   
wt amplicon (over InsPos) 06gc - 65cs
wt amplicon start Chr1:18863694
wt amplicon end Chr1:18864636
wt amplicon size 943 bp
Locus specific primer 65cs: 5'-GGCATGTCGTTTTGATAAGGTACT-3'
2nd locus specific primer 06gc: 5'-CACAAACCATTTGACCTACTCCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11