Genotyping and primer details


Genotyping and primer details for SALK_143098c - At1g04510
Line ID SALK_143098c
Locus At1g04510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1226896 of line SALK_143098c Chr1:1226896
   
Insertion allele amplicon LBb3 - 03bz
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 03bz: 5'-TGATTTGATGAATCGATACTTTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1225963
   
wt amplicon (over InsPos) 18da - 03bz
wt amplicon start Chr1:1225963
wt amplicon end Chr1:1227181
wt amplicon size 1219 bp
Locus specific primer 03bz: 5'-TGATTTGATGAATCGATACTTTGG-3'
2nd locus specific primer 18da: 5'-AATAACTCCAGTGGTTGCATTACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11