Genotyping and primer details


Genotyping and primer details for SALK_143488c - At1g01350
Line ID SALK_143488c
Locus At1g01350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:137577 of line SALK_143488c Chr1:137577
   
Insertion allele amplicon LBb3 - 44ux
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 44ux: 5'-TTGAAAGGGAATAAGAAAAAGGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:137276
   
wt amplicon (over InsPos) 68vl - 44ux
wt amplicon start Chr1:137276
wt amplicon end Chr1:138206
wt amplicon size 931 bp
Locus specific primer 44ux: 5'-TTGAAAGGGAATAAGAAAAAGGCT-3'
2nd locus specific primer 68vl: 5'-AATCAGGATATCGAGTTTGACGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11