Genotyping and primer details


Genotyping and primer details for SALK_143654c - At3g50650
Line ID SALK_143654c
Locus At3g50650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18807546 of line SALK_143654c Chr3:18807546
   
Insertion allele amplicon LBb3 - AD10
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer AD10: 5'-CACTCTCCATTGTTCTTTCTCCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18806652
   
wt amplicon (over InsPos) 06cd - AD10
wt amplicon start Chr3:18806652
wt amplicon end Chr3:18807724
wt amplicon size 1073 bp
Locus specific primer AD10: 5'-CACTCTCCATTGTTCTTTCTCCTC-3'
2nd locus specific primer 06cd: 5'-CTTCATATCTCAACCGAGTCAACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11