Sequences from confirmation amplicon


Confirmation sequences for line SALK_143654c and hit At3g50650 are shown.
Line ID SALK_143654c
Locus At3g50650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18807546
Primers used for PCR AD10 - LBb3, show primer details
   
Sequence Name 84-K107675-SALK_143654C-AD10
GenBank Accession LN486268 [GenBank]
Sequencing primer gene specific primer, AD10: 5'-CACTCTCCATTGTTCTTTCTCCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K107675-SALK_143654C-AD10
GTTATTATATCATCATCTGATCGGACCAAATCCATAATCCTCCTACCGAACAACACTCTC
TCCACTCTCAGCCTTTCTTTCGAATCTCGATCCAAATTCGGTTCAAGCGATTCAAAAACC
GCGGAATAAAACCGGAGAGAGTTCTTAACCCGGTTAGCAAATTCAACCCGGTTTAAACTG
ACTTCGTATTCTCCAAGCGTCACAATCCTCGGGTTTAACGATCTAGCTAACCGGAGCGCC
GTACCAACAGTCGTCGCTGTTTCATCTAGAAGCTTATAAAGCTCAAGCATAAAATTCACA
ACCAAAACCTCATCCGGGTCAACCCGAAAACTTGACCCGTTTAGTAATTGAATCGGAGTC
AGAACCGGATAAAACTCGAAATTGAGATCCAAAATCGCCGCGAAATCACGGAGACGGTTT
CCCGTAGCGATCAGAGACGGTCCCGGTGAGTCTCCTAGAGACGGAGCGGGTATACCCGAA
ATCCGGATCCGGGTGGGTTTACCAGAAGAACGGGTCGCTAAAGCTTGTAACAGAGCAGAC
CATTGAATACCTTGAAAAATCCCGAAATCGACTATGTGAATGTTGTTTGATTGATTCGTA
GCTTCGAGAATTGCTTGATTCGCTGTTAAATGAGCGAACTTTGAATATGGACATGCGTCG
TTTAAGGTTTTGTATGAGAGAATGAAATCTTCTAGGGATGACGACGACGATGACGACGGA
GACTCTGTTTCTTTATGAGACAGAGCTTCTGCGAAGTAATACCCGACTCGTTGTATCGGG
TCGCCTGATTCGGACACGGATTCTTTGATCCGAATTAGTGTGTCCGGTTTAGTTTCCGGT
TTACGTTTATATTGTGGTGTAAACAAATTGACGTTTAGACAACTTAATAACACATTGCGG
ACGTTTTTATGTACTGGGGTGGTTTTTCTTTTACCAGTGAGACGGGNCACAGCTGATTGC
CCTTCACGCC
   
Sequence Name 82-K107674-SALK_143654C-R210
GenBank Accession LN486241 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K107674-SALK_143654C-R210
CGTCATTTGTTTACCCCAATATAAACGTAAACCGGAAACTAAACCGGACACACTACTTCG
GATCAAAGAATCCGTGTCCGAATCAGGCGACCCGATACAACGAGTCGGGTATTACTTCGC
AGAAGCTCTGTCTCATAAAGAAACAGAGTCTCCGTCGTCATCGTCGTCGTCATCCCTAGA
AGATTTCATTCTCTCATACAAAACCTTAAACGACGCATGTCCATATTCAAAGTTCGCTCA
TTTAACAGCGAATCAAGCAATTCTCGAAGCTACGAATCAATCAAACAACATTCACATAGT
CGATTTCGGGATTTTTCAAGGTATTCAATGGTCTGCTCTGTTACAAGCTTTAGCGACCCG
TTCTTCTGGTAAACCCACCCGGATCCGGATTTCGGGTATACCCGCTCCGTCTCTAGGAGA
CTCACCGGGACCGTCTCTGATCGCTACGGGAAACCGTCTCCGTGATTTCGCGGCGATTTT
GGATCTCAATTTCGAGTTTTATCCGGTTCTGACTCCGATTCAATTACTAAACGGGTCAAG
TTTTCGGGTTGACCCGGATGAGGTTTTGGTTGTGAATTTTATGCTTGAGCTTTATAAGCT
TCTAGATGAAACAGCGACGACTGTTGGTACGGCGCTCCGGTTAGCTAGATCGTTAAACCC
GAGGATTGTGACGCTTGGAGAATACGAAGTCAGTTTAAACCGGGTTGAATTTGCTAACCG
GGTTAAGAACTCTCTCCGGTTTTATTCCGCGGTTTTTGAATCGCTTGAACCGAATTTGGA
TCGAGATTCGAAAGAAAGGCTGAGAGTGGAGAGAGTGTTGTTCGGTAGGAGGATTATGGA
TTTGGTCCGATCAGATGATGATAATAATAAACCGGGAACCCGGTTTGGGTTAATGGAGGA
G

Last Updated on 06.12.2018 16:11