Genotyping and primer details


Genotyping and primer details for SALK_143876c - At2g39690
Line ID SALK_143876c
Locus At2g39690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:16540825 of line SALK_143876c Chr2:16540825
   
Insertion allele amplicon LBb3 - 41oh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 41oh: 5'-CACGATGGCTAGTTCTTGTATGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:16541395
   
wt amplicon (over InsPos) 01px - 41oh
wt amplicon start Chr2:16540484
wt amplicon end Chr2:16541395
wt amplicon size 912 bp
Locus specific primer 41oh: 5'-CACGATGGCTAGTTCTTGTATGAG-3'
2nd locus specific primer 01px: 5'-TTTCCGTATGCAAATTTTCTTTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11