Genotyping and primer details

Genotyping and primer details for SALK_144061c - At5g14680
Line ID SALK_144061c
Locus At5g14680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:4731819 of line SALK_144061c Chr5:4731819
Insertion allele amplicon LBb3 - 43vi
Locus specific primer 43vi: 5'-TACAGCAGACCAGACATTGGATAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:4731308
wt amplicon (over InsPos) 52vx - 43vi
wt amplicon start Chr5:4731308
wt amplicon end Chr5:4732615
wt amplicon size 1308 bp
Locus specific primer 43vi: 5'-TACAGCAGACCAGACATTGGATAG-3'
2nd locus specific primer 52vx: 5'-AGTCAAAGAACCGACAAGAACTGT-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11