Sequences from confirmation amplicon


Confirmation sequences for line SALK_144061c and hit At5g14680 are shown.
Line ID SALK_144061c
Locus At5g14680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4731819
Primers used for PCR 43vi - LBb3, show primer details
   
Sequence Name 61-K117993-SALK_144061C-43vi
GenBank Accession LN485777 [GenBank]
Sequencing primer gene specific primer, 43vi: 5'-TACAGCAGACCAGACATTGGATAG-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K117993-SALK_144061C-43vi
CTAGATTACCACAAATAAAGGAACTTTTTGTTTTTATGTTTCGAGAATGGAGACTAAACA
CGAATATATATGATGCTTTGTGTGTGTAGTATCATCGAATAACATCTAGAATTACAGATG
CACTTACTAGATCAATCAAAAGAGTTTTTTGTAACGTTTTTCTTCTTCTGTACTGCAACA
CACCCATTTATTGTCTCTCAAAAGCTTGCGGGATTCAGCAGACATGAAATCACCGCATGG
TTTTGCGTTGCTTTTGAAATTTAGTCATCAGCTGGATCTTGTGGAGATTCTTCAGCAGTG
CGTTTGATTGTGATGACTGGGCATTCTGCATGCTTCACACAAAATTCACTCACTGTTCCA
ACAAATACCCTGTCCAGTGGTAAACAAATGTGAACTTGATCAATATCTGTGATTTCAAGC
TCAAGCAAGTACAAGAGCTAATGACGATTCTCGGTGCAGTATAACACATAACACATTGAC
GCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTT
TCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCA
GCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGA
AATGGGCAAAA
   
Sequence Name 50-K117992-SALK_144061C-R210
GenBank Accession LN485518 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K117992-SALK_144061C-R210
GTGTTAACTGCACCGAGAATCGTCATTAGCTCTTGTACTTGCTTGAGCTTGAAATCACAG
ATATTGATCAAGTTCACATTTGTTTACCACTGGACAGGGTATTTGTTGGAACAGTGAGTG
AATTTTGTGTGAAGCATGCAGAATGCCCAGTCATCACAATCAAACGCACTGCTGAAGAAT
CTCCACAAGATCCAGCTGATGACTAAATTTCAAAAGCAACGCAAAACCATGCGGTGATTT
CATGTCTGCTGAATCCCGCAAGCTTTTGAGAGACAATAAATGGGTGTGTTGCAGTACAGA
AGAAGAAAAACGTTACAAAAAACTCTTTTGATTGATCTAGTAAGTGCATCTGTAATTCTA
GATGTTATTCGATGATACTACACACACAAAGCATCATATATATTCGTGTTTAGTCTCCAT
TCTCGAAACATAAAAACAAAAAGTTCCTTTATTTGTTGTGTAATCTTAGACTCTTCAATG
TCTTTCATATATGGCTATCCAATGTCTGGCCTGCTGTA

Last Updated on 06.12.2018 16:11