Genotyping and primer details


Genotyping and primer details for SALK_144108c - At1g74830
Line ID SALK_144108c
Locus At1g74830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28114395 of line SALK_144108c Chr1:28114395
   
Insertion allele amplicon LBb3 - 51wx
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 51wx: 5'-TATGAAACTCTTCCCTTGCATCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28113948
   
wt amplicon (over InsPos) 02xf - 51wx
wt amplicon start Chr1:28113948
wt amplicon end Chr1:28115143
wt amplicon size 1196 bp
Locus specific primer 51wx: 5'-TATGAAACTCTTCCCTTGCATCTT-3'
2nd locus specific primer 02xf: 5'-AGCCACAATGTCTAAACGTTCTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11