Sequences from confirmation amplicon


Confirmation sequences for line SALK_144108c and hit At1g74830 are shown.
Line ID SALK_144108c
Locus At1g74830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28114395
Primers used for PCR 51wx - LBb3, show primer details
   
Sequence Name 31-K118859-SALK_144108C-51wx
GenBank Accession LN485158 [GenBank]
Sequencing primer gene specific primer, 51wx: 5'-TATGAAACTCTTCCCTTGCATCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K118859-SALK_144108C-51wx
ACCTCAATTCAGCTTCAAGCTCCTTCATTTCCTCTTCCCTCTTCGCCAATTCACTACTCA
TAGACTGCAAAGCTTCTTGGTCATACTCTGCTTGTTCATCCATCATTCTTTGGTACTGCA
AAGCCTCCATTTGAACAGCAGCTTTCTCGGCTTGAAGCCTCGTGATCATAGCCATCGCTT
CGTTGGCTGCAACAGCTGAAGCACTTCTCTCTTCATCTAACTCCATATATAAATCAATGA
GTGACTTCTTGTCCAAGCGAACCTCTTTCTTGAGCTGATTAAGAATGGATTCACCGGTTG
GATCTGTCGTGTCCGAAGCATTCTCTGTCTTATTCAAAACGGATTTCTTAAGAGATCGAA
CGGACCATCGTGGACTGTTTTGCGCCGAATCAAATTGACGCTTAGACAACTTAATAACAC
ATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACA
GCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTT
GCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCAAATCGGGCAAAA
   
Sequence Name 07-K118858-SALK_144108C-R210
GenBank Accession LN484477 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K118858-SALK_144108C-R210
GTCATTTGATCGGCGCAAACAGTCCACGATGGTCCGTTCGATCTCTTAAGAAATCCGTTT
TGAATAAGACAGAGAATGCTTCGGACACGACAGATCCAACCGGTGAATCCATTCTTAATC
AGCTCAAGAAAGAGGTTCGCTTGGACAAGAAGTCACTCATTGATTTATATATGGAGTTAG
ATGAAGAGAGAAGTGCTTCAGCTGTTGCAGCCAACGAAGCGATGGCTATGATCACGAGGC
TTCAAGCCGAGAAAGCTGCTGTTCAAATGGAGGCTTTGCAGTACCAAAGAATGATGGATG
AACAAGCAGAGTATGACCAAGAAGCTTTGCAGTCTATGAGTAGTGAATTGGCGAAGAGGG
AAGAGGAAATGAAGGAGCTTGAAGCTGAATTTGAGGTTTACAGAGAGAAATATGGATGTT
TGACTGATCAAGAAGATGCAAGGGAAGAGTTTCAT

Last Updated on 06.12.2018 16:11