Genotyping and primer details


Genotyping and primer details for SALK_144401 - At3g53960
Line ID SALK_144401
Locus At3g53960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:19980718 of line SALK_144401 Chr3:19980718
   
Insertion allele amplicon LBb3 - 19lu
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 19lu: 5'-GTCCAAAAGGTACTACCGATCAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:19980146
   
wt amplicon (over InsPos) 03na - 19lu
wt amplicon start Chr3:19980146
wt amplicon end Chr3:19981033
wt amplicon size 888 bp
Locus specific primer 19lu: 5'-GTCCAAAAGGTACTACCGATCAAA-3'
2nd locus specific primer 03na: 5'-AAGCACTATGGAGGAAGAGTCAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11