Sequences from confirmation amplicon


Confirmation sequences for line SALK_144401 and hit At3g53960 are shown.
Line ID SALK_144401
Locus At3g53960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19980718
Primers used for PCR 19lu - LBb3, show primer details
   
Sequence Name 47-K114227-SALK_144401C-19lu
GenBank Accession LN485436 [GenBank]
Sequencing primer gene specific primer, 19lu: 5'-GTCCAAAAGGTACTACCGATCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K114227-SALK_144401C-19lu
AATATTATAATATAAATATAGATTATATATTAATAAGGTAGATATATAAATATATATCTA
AAATTTTGAACTCATGATATTTTTGGGTATCATTGACTCGGTTATGAGAGTATAGAAGAG
GTGTGTATATGTGAAGGTCTATCTTTAAACTATTGAAAAGGATCGATAATAGTTTTTGAG
TATTATTATTTATCCGGTTTCTCAGAAATTAGTACCATAAGGTAAATGGTGGTTGCAAGT
AGGACAGTTCCATAACGGCCGAGATAAGCATCTGCGACGAAGCCTCCAAGAAGAGGCATC
AAAGTAGTGACACCAGACCAGTAGTTCGTATTTTTTACAGCCATCTTGAGATCTTGGTGA
AGAATGGTAGTCAAGTAGACCACTAAGTTCGTGGAGATCCCAAAGTAACTTAGCCTCTCG
CTGAATTCGATACCTTTATGCATATATCANACCATAAGACTATNACGTAAAAAATACTTC
ACACATAAAGTTATTTTATAGATATAATTCAAAAAAATCTCTTTAGAACAAATTGACGCT
NAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCA
CCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCA
AGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 43-K114226-SALK_144401C-R210
GenBank Accession LN485355 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K114226-SALK_144401C-R210
TCTAAGAGATTTTTTTGAATTTATCTATAAAATAACTTTATGTGTGAAGTATTTTTTACG
TTATAGTCTTATGGTTTGATATATGCATAAAGGTATCGAATTCAGCGAGAGGCTAAGTTA
CTTTGGGATCTCCACGAACTTAGTGGTCTACTTGACTACCATTCTTCACCAAGATCTCAA
GATGGCTGTAAAAAATACGAACTACTGGTCTGGTGTCACTACTTTGATGCCTCTTCTTGG
AGGCTTCGTCGCAGATGCTTATCTCGGCCGTTATGGAACTGTCCTACTTGCAACCACCAT
TTACCTTATGGTACTAATTTCTGAGAAACCGGATAAATAATAATACTCAAAAACTATTAT
CGATCCTTTTCAATAGTTTAAAGATAGACCTTCACATATACACACCTCTTCTATACTCTC
ATAACCGAGTCAATGATACCCAAAAATATCAGGAGTTCAAAATTTTAAATATATATNTAT
ATATCTACCTTATTAATATATATATCTATATTTTATATTTTATAATTTATTTTTGTTATT
TTGTTGTCGGTTTTGATCGGTA

Last Updated on 06.12.2018 16:11