Genotyping and primer details


Genotyping and primer details for SALK_144987 - At1g79210
Line ID SALK_144987
Locus At1g79210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29796320 of line SALK_144987 Chr1:29796320
   
Insertion allele amplicon LBb3 - 79hw
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 79hw: 5'-TTGTAATGTTTGACGGATTGTCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29795400
   
wt amplicon (over InsPos) 02kj - 79hw
wt amplicon start Chr1:29795400
wt amplicon end Chr1:29796668
wt amplicon size 1269 bp
Locus specific primer 79hw: 5'-TTGTAATGTTTGACGGATTGTCAT-3'
2nd locus specific primer 02kj: 5'-TGGAAGCATGTCATTTCATCTTAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11