Genotyping and primer details


Genotyping and primer details for SALK_145035c - At5g48850
Line ID SALK_145035c
Locus At5g48850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19806869 of line SALK_145035c Chr5:19806869
   
Insertion allele amplicon R204 - AW35
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer AW35: 5'-ACCATTGAAAGCTTCTCCTTGATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19806158
   
wt amplicon (over InsPos) 20lt - AW35
wt amplicon start Chr5:19806158
wt amplicon end Chr5:19807165
wt amplicon size 1008 bp
Locus specific primer AW35: 5'-ACCATTGAAAGCTTCTCCTTGATA-3'
2nd locus specific primer 20lt: 5'-CGCGGAATTAATATCCACTTATTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11