Sequences from confirmation amplicon


Confirmation sequences for line SALK_145035c and hit At5g48850 are shown.
Line ID SALK_145035c
Locus At5g48850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19806869
Primers used for PCR AW35 - R204, show primer details
   
Sequence Name 23-K106869-SALK_145035C-R210
GenBank Accession LN484935 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K106869-SALK_145035C-R210
CCAAAAAAGAAAAGTTATTGTTCCAATTTGTTGAACAAAAAAAAGTTGTTCCAATTTATG
GTCTAGATGTTGCCGTGTGCGTATGAGAGTCAGTCGTTGCACCGAACGGTGTGGGATCTC
GTTTACTTTTTCCGAATAATAGTGCAAAAAGTCTTTTACGTTTGAAAGAATATAATTCTT
CACATTTTTTATTGGCCGGCCCATCGAATCATATCCTAATTGGTCAAATACATGAATATA
TTTCTATATGAGAAGAGTAAAGTAATTGGTTATTAATAATTAGTATGTCTTAATGTTGGT
TGCAGTTGATAGAGAAGAACCCGGAGATGGCAATAGTGTGGTTTTGGAAAGCCATCAACA
CTGGAGACAGAGTAGACAGTGCCCTCAAGGACATGGCTGTTGTAATGAAACAACTTGACC
GTTCTGAAGAAGCTATTGAAGCTATCAAATCTTTCCGTCCTCGTTGTTCCAAGAATTCCC
AAGATTCCCTTGACAATGTCCTCATCGACTTATACAAGGTCTTACTATAACCATACACGC
TAACATACTCTATTTGTGGAATTCAGTATATAAAAATGATAATAGTCCTTTATTATGACA
CTGACATAGCGCAATTTCTTACTATGAATAGAAATGTGGGAGAATGGAGGAGCAAGTTGA
GTTGTTGAAGAGGAAATTAAGGCAGATCTATCAAGGAG
   
Sequence Name 26-K106870-SALK_145035C-AW35
GenBank Accession LN485021 [GenBank]
Sequencing primer gene specific primer, AW35: 5'-ACCATTGAAAGCTTCTCCTTGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K106870-SALK_145035C-AW35
CCTCCATTCTCCCCATTTCTATTCATAGTAAGAAATTGCGCTATGTCAGTGTCATAATAA
AGGACTATTATCATTTTTATATACTGAATTCCACAAATAGAGTATGTTAGCGTGTATGGT
TATAGTAAGACCTTGTATAAGTCGATGAGGACATTGTCAAGGGAATCTTGGGAATTCTTG
GAACAACGAGGACGGAAAGATTTGATAGCTTCAATAGCTTCTTCAGAACGGTCAAGTTGT
TTCATTACAACAGCCATGTCCTTGAGGGCACTGTCTACTCTGTCTCCAGTGTTGATGGCT
TTCCAAAACCACACTATTGCCATCTCCGGGTTCTTCTCTATCAACTGCAACCAACATTAA
GACATACTAATTATTAATAACCAATTACTTTACTCTTCTCATATAGAAATATATTCATGT
ATTTGACCAATTAGGATATGATTCGATGGGCCGGCCAATAAAAAATGTGAAGAATTATAT
TCTTTCAAACGTAAAAGACTTTTTGCACTATTATTCGGAAAAAGTAAACGAGATCCCACA
CCGTTCGGTGCAACGACTGACTCTCATACGCACACGGCAACATCTAGACCATAAATTGGA
ACAACTTTTTTTTGTTCAACAAATTGGAACAATATACTTCTTCTTTTTTTTTGGCTTAGA
CAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAG
TGAGACGGGCAACAGCTGATTGCCCTTCACCGCCGGGCCCTGAGAGAGTTGCACA

Last Updated on 06.12.2018 16:11