Genotyping and primer details


Genotyping and primer details for SALK_145495 - At1g54170
Line ID SALK_145495
Locus At1g54170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20222506 of line SALK_145495 Chr1:20222506
   
Insertion allele amplicon LBb3 - 17jd
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 17jd: 5'-TTCGGACTAAGCTGTGTTGTATTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20221675
   
wt amplicon (over InsPos) 13km - 17jd
wt amplicon start Chr1:20221675
wt amplicon end Chr1:20223028
wt amplicon size 1354 bp
Locus specific primer 17jd: 5'-TTCGGACTAAGCTGTGTTGTATTG-3'
2nd locus specific primer 13km: 5'-GAAACCAAATACTCATCGGTCTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11