Genotyping and primer details


Genotyping and primer details for SALK_146290 - At1g04540
Line ID SALK_146290
Locus At1g04540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1238634 of line SALK_146290 Chr1:1238634
   
Insertion allele amplicon LBb3 - 48dr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 48dr: 5'-AAGATCACACAAAGCTCAGATCGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1239065
   
wt amplicon (over InsPos) 10ep - 48dr
wt amplicon start Chr1:1238324
wt amplicon end Chr1:1239065
wt amplicon size 742 bp
Locus specific primer 48dr: 5'-AAGATCACACAAAGCTCAGATCGT-3'
2nd locus specific primer 10ep: 5'-AACATTATCGCATCTACTCCGATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11