Sequences from confirmation amplicon


Confirmation sequences for line SALK_146290 and hit At1g04540 are shown.
Line ID SALK_146290
Locus At1g04540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1238634
Primers used for PCR 48dr - LBb3, show primer details
   
Sequence Name 78-K110311-SALK_146290C-48dr
GenBank Accession LN486186 [GenBank]
Sequencing primer gene specific primer, 48dr: 5'-AAGATCACACAAAGCTCAGATCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K110311-SALK_146290C-48dr
TCCGGCTCACCTTTCTAGTAGATCCTTCTGAGACCATCGGCCCACCACCACCACCGCATA
CGAAACTGCATTCCACACCACAAATCTTACTAAAACACGAGAAAAGTCCATTACCTCCAT
CTGTGTGACGGCGGTTATGCTTTTTCGTCGTCGGAGTTTTCCGTCTTGAACTCATTCCTC
CCCCGGCAGTTGCAGCTGGAACGCTCGCGCCATCGTAATCAGTGCTACTTATATGGCTTG
ATCCTATGTCGTACAACGGTGGAAGCTCTGTTCGCCATCTCTCGAGTTTTGATCGCAATC
CTTCGATGCTAGATTCATCGATGCTCCATTCGCTGAGTATTGAACTTTCCGTTTCGTGCG
ATCTATCTTTCGCCAATTTGTGTTATCCAGGATATATTGTGGTGTAAACAAATTGACGCT
TAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCA
CCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCA
AGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAAT
   
Sequence Name 68-K110310-SALK_146290C-R210
GenBank Accession LN485949 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K110310-SALK_146290C-R210
CGTCATTTGTTTCACCACAATATATCCTGGATAACACAAATTGGCGAAAGATAGATCGCA
CGAAACGGAAAGTTCAATACTCAGCGAATGGAGCATCGATGAATCTAGCATCGAAGGATT
GCGATCAAAACTCGAGAGATGGCGAACAGAGCTTCCACCGTTGTACGACATAGGATCAAG
CCATATAAGTAGCACTGATTACGATGGCGCGAGCGTTCCAGCTGCAACTGCCGGGGGAGG
AATGAGTTCAAGACGGAAAACTCCGACGACGAAAAAGCATAACCGCCGTCACACAGATGG
AGGTAATGGACTTTTCTCGTGTTTTAGTAAGATTTGTGGTGTGGAATGCAGTTTCGTATG
CGGTGGTGGTGGTGGGCCGATGGTCTCAGAAGGATCTACTAAGAAAGGTGGAGCCGGACG
TGTGCAACGCACATATTCCGCTGACGATCTGAGCTTTGTGTGATCT

Last Updated on 06.12.2018 16:11