Genotyping and primer details


Genotyping and primer details for SALK_147280c - At1g16500
Line ID SALK_147280c
Locus At1g16500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5639814 of line SALK_147280c Chr1:5639814
   
Insertion allele amplicon LBb3 - 48um
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 48um: 5'-CTAACCCGTGACTTAAGTTGCTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5640364
   
wt amplicon (over InsPos) 56vr - 48um
wt amplicon start Chr1:5639380
wt amplicon end Chr1:5640364
wt amplicon size 985 bp
Locus specific primer 48um: 5'-CTAACCCGTGACTTAAGTTGCTTC-3'
2nd locus specific primer 56vr: 5'-ACAAAACAGAGAGGTTTCCAAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11