Genotyping and primer details


Genotyping and primer details for SALK_147811c - At1g03210
Line ID SALK_147811c
Locus At1g03210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:784027 of line SALK_147811c Chr1:784027
   
Insertion allele amplicon LBb3 - 62bk
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 62bk: 5'-GGAATTGTTCGGTTGACTTGTTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:783207
   
wt amplicon (over InsPos) 01cx - 62bk
wt amplicon start Chr1:783207
wt amplicon end Chr1:784198
wt amplicon size 992 bp
Locus specific primer 62bk: 5'-GGAATTGTTCGGTTGACTTGTTAT-3'
2nd locus specific primer 01cx: 5'-TCTCAGTAGAACCCTCTGCTTCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11