Sequences from confirmation amplicon


Confirmation sequences for line SALK_147811c and hit At1g03210 are shown.
Line ID SALK_147811c
Locus At1g03210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:784072
Primers used for PCR 62bk - LBb3, show primer details
   
Sequence Name 24-K108287-SALK_147811C-62bk
GenBank Accession LN484967 [GenBank]
Sequencing primer gene specific primer, 62bk: 5'-GGAATTGTTCGGTTGACTTGTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K108287-SALK_147811C-62bk
TCATTCACTGGCTCTTGTGAATCTCTCTTTGCTTTGCTGTTATTGTGCAGGTGGATTTGT
GTGGTCATGCAACTTTAGCATCTGCTCACGTTCTCTTCTCAACTGGTTTGGTTGGTTCAG
AGACAGTTGAGTTCGACACATTATCAGGGATTCTAACAGCTAAACATCTCAAAAATGATG
ATGATGGAGAAGAATCAAGTATCGAATTGGATTTCCCTGTTGTTCCAACATATGAAGTCA
ACTACATTGATGATGATCTCTCTTTGTTCTCCAAAGCCTTGAATGGAGCTACCATTCTTG
ATGTCAAAGCCACAAAGAAAGACCTCCTCGTAACTATATTTCACCAACTGTCTCCATTTT
TCCTATTCTTGCTATGGGAACCAAAATCTCGATTCTTTTCGAATGGTTTGCAGGTTGTGC
TTTCATCTTGGGAAGCTGTTATTGATTTGAAGCCAAGATTAGATGAGATATCGAAATGCC
CTTGTGAAGGAATGATGGTGACTGCCGCTGCTTCTGATGGATCTACTTATGATTTCTGTA
GTCGCTACTTTGCCCCGAGATTCGGAATCAATGAGGTTATCATTTAGTCTTTTGCTCTTA
GAAGATTAAGAGCGAGTGGTCAAAACAGCAAAAACGTGACCTCTTTTGCTTTCTTTTTAC
TGTGTGGCTCTTCAGGACCCTGTTACTGGAAGTGCTCATTGTGCTTTAGCTCATTACTGG
AGCCTCAGGATGAACAAGTGTGATTTCTTCGCCTACCAGGTTCTCTCTCTCTTTCTCTCC
ACGCAGACACTTAAAAGACAAATTCTGAACAAATTGACGCTTAGACAACTTAATAACACA
TTGCGGAACGTTTTAATGTACTGGGGTGGTTTTTCTTTTCACAAGTGAGACGGGCAACAG
CTGATTGCCCTTCACGCCCTGGCCCTG
   
Sequence Name 08-K108286-SALK_147811C-R210
GenBank Accession LN484481 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K108286-SALK_147811C-R210
GTCATTTGTTCGAATTTGTCTTTTACTGTCTGCGTGGAGAGAAAGAGAGAGAGCCCTTAT
AGGCGAAGAAATCACACTTGTTCATCCTGAGGCTCCCGTTATGAGCTAAAGCACAATGAG
CACTTCCAGTAACAGGGTCCTGAAGAGCCACACAGTAAAAAGAAAGCAAAAGAGGTCACG
TTTTTGCTGTTTTGACCACTCGCTCTTAATCTTCTAAGAGCAAAAGACTAAATGATAACC
TCATTGATTCCGAATCTCGGGGCAAAGTAGCGACTACAGAAATCATAAGTAGATCCATCA
GAAGCAGCGGCAGTCACCATCATTCCTTCACAAGGGCATTTCGATATCTCATCTAATCTT
GGCTTCAAATCAATAACAGCTTCCCAAGATGAAAGCACAACCTGCAAACCATTCGAAAAG
AATCGAGATTTTGGTTCCCATAGCAAGAATAGGAAAAATGGAGACAGTTGGTGAAATATA
GTTACGAGGAGGTCTTTCTTTGTGGCTTTGACATCAAGAATGGTAGCTCCATTCAAGGCT
TTGGAGAACAAAGAGAGATCATCATCAATGTAGTTGACTTCATATGTTGGAACAACAGGG
AAATCCAATTCGATACTTGATTCTTCTCCATCATCATCATTTTTGAGATGTTTAGCTGTT
AGAATCCCTGATAATGTGTCGAACTCAACTGTCTCTGAACCAACCAAACCAGTTGAGAAG
AGAACGTGAGCAGATGCTAAAGTTGCATGACCACACAAATCCACCTGCACAATAACAGCA
AAGCAAAGAGAGATTCACAAGAGCCAGTGAATGAGTACAAGTTCTAGTTATCAGGATCTA
TCAAGTCAATGATAACAAGTCAACCGAACAAT

Last Updated on 06.12.2018 16:11