Genotyping and primer details


Genotyping and primer details for SALK_148625c - At3g59140
Line ID SALK_148625c
Locus At3g59140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21864462 of line SALK_148625c Chr3:21864462
   
Insertion allele amplicon LBb3 - 49um
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 49um: 5'-GGTATGGCGCTATCTTATGGTTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21864776
   
wt amplicon (over InsPos) 55vr - 49um
wt amplicon start Chr3:21863645
wt amplicon end Chr3:21864776
wt amplicon size 1132 bp
Locus specific primer 49um: 5'-GGTATGGCGCTATCTTATGGTTTA-3'
2nd locus specific primer 55vr: 5'-AAAACCCATCAAAAACAGATTCAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11