Genotyping and primer details


Genotyping and primer details for SALK_148834c - At5g52540
Line ID SALK_148834c
Locus At5g52540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:21321669 of line SALK_148834c Chr5:21321669
   
Insertion allele amplicon R204 - SC10
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer SC10: 5'-GCTACTGGAATTGCTGTGTCATTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21322247
   
wt amplicon (over InsPos) 10cg - SC10
wt amplicon start Chr5:21321239
wt amplicon end Chr5:21322247
wt amplicon size 1009 bp
Locus specific primer SC10: 5'-GCTACTGGAATTGCTGTGTCATTA-3'
2nd locus specific primer 10cg: 5'-CAATCTTGAGATGGTACAAGGACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11