Genotyping and primer details


Genotyping and primer details for SALK_149104 - At1g08940
Line ID SALK_149104
Locus At1g08940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2878785 of line SALK_149104 Chr1:2878785
   
Insertion allele amplicon LBb3 - 68us
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 68us: 5'-GTGAATTTCAATTCGATCTTGGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2879224
   
wt amplicon (over InsPos) 67vl - 68us
wt amplicon start Chr1:2878335
wt amplicon end Chr1:2879224
wt amplicon size 890 bp
Locus specific primer 68us: 5'-GTGAATTTCAATTCGATCTTGGAT-3'
2nd locus specific primer 67vl: 5'-GTTTGATTGGTTTTCGAATTTGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11