Genotyping and primer details


Genotyping and primer details for SALK_150134c - At3g60630
Line ID SALK_150134c
Locus At3g60630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22410854 of line SALK_150134c Chr3:22410854
   
Insertion allele amplicon LBb3 - 25lu
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 25lu: 5'-CTTAACAACAACAACGACGACACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22411539
   
wt amplicon (over InsPos) 08rd - 25lu
wt amplicon start Chr3:22410468
wt amplicon end Chr3:22411539
wt amplicon size 1072 bp
Locus specific primer 25lu: 5'-CTTAACAACAACAACGACGACACT-3'
2nd locus specific primer 08rd: 5'-AACCGTGAAAATATCCTCCTTTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11