Genotyping and primer details


Genotyping and primer details for SALK_150343c - At3g10630
Line ID SALK_150343c
Locus At3g10630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3321811 of line SALK_150343c Chr3:3321811
   
Insertion allele amplicon LBb3 - 06gj
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 06gj: 5'-TGTTGATGTTGATATAGGGTGGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3320920
   
Insertion allele amplicon LBb3 - 18yk
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 18yk: 5'-GTTGTTTGTCATAGTGAGCCTGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3322705
   
Insertion allele amplicon LBb3 - 69yq
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 69yq: 5'-TAGCTAAGGAGACGAAGGAGGTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3322773
   
Insertion allele amplicon LBb3 - PP04
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer PP04: 5'-AAGGTTGGGATGTATTGTTGAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3322326
   
wt amplicon (over InsPos) 06hs - 06gj
wt amplicon start Chr3:3320920
wt amplicon end Chr3:3321848
wt amplicon size 929 bp
Locus specific primer 06gj: 5'-TGTTGATGTTGATATAGGGTGGAA-3'
2nd locus specific primer 06hs: 5'-TGATGAGGCTAAGGTTAAAGGAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11