Genotyping and primer details


Genotyping and primer details for SALK_150349 - At3g07740
Line ID SALK_150349
Locus At3g07740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2472489 of line SALK_150349 Chr3:2472489
   
Insertion allele amplicon LBb3 - 55gp
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 55gp: 5'-AGCGGGGAGCTAGAGTAAATAAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2471903
   
wt amplicon (over InsPos) 20hs - 55gp
wt amplicon start Chr3:2471903
wt amplicon end Chr3:2472838
wt amplicon size 936 bp
Locus specific primer 55gp: 5'-AGCGGGGAGCTAGAGTAAATAAAC-3'
2nd locus specific primer 20hs: 5'-TCATACTGCTGTCGTCTCTTGTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11