Genotyping and primer details


Genotyping and primer details for SALK_150833c - At3g14210
Line ID SALK_150833c
Locus At3g14210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4730615 of line SALK_150833c Chr3:4730615
   
Insertion allele amplicon LBb3 - 34cy
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 34cy: 5'-AAGACGGTGAACTGAAAACCATAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4731074
   
wt amplicon (over InsPos) 27dw - 34cy
wt amplicon start Chr3:4729988
wt amplicon end Chr3:4731074
wt amplicon size 1087 bp
Locus specific primer 34cy: 5'-AAGACGGTGAACTGAAAACCATAG-3'
2nd locus specific primer 27dw: 5'-GTAGCTTTGTTCACTTTCGGAGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11