Genotyping and primer details


Genotyping and primer details for SALK_150899c - At4g31920
Line ID SALK_150899c
Locus At4g31920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15444356 of line SALK_150899c Chr4:15444356
   
Insertion allele amplicon LBb3 - 10bt
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 10bt: 5'-TTCAGATCAACAACAACATCAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15445023
   
wt amplicon (over InsPos) 05da - 10bt
wt amplicon start Chr4:15444090
wt amplicon end Chr4:15445023
wt amplicon size 934 bp
Locus specific primer 10bt: 5'-TTCAGATCAACAACAACATCAACA-3'
2nd locus specific primer 05da: 5'-TGACTTCCTCTTCAAAACACCTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11