Genotyping and primer details


Genotyping and primer details for SALK_151040 - At4g27460
Line ID SALK_151040
Locus At4g27460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13733635 of line SALK_151040 Chr4:13733635
   
Insertion allele amplicon LBb3 - CE43
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CE43: 5'-CATGTCATTCTCCAAAAATTTCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13734260
   
Insertion allele amplicon LBb3 - CE44
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CE44: 5'-CCTTAAACTCATCCGTCTCTGTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13733192
   
wt amplicon (over InsPos) CE44 - CE43
wt amplicon start Chr4:13733192
wt amplicon end Chr4:13734260
wt amplicon size 1069 bp
Locus specific primer CE43: 5'-CATGTCATTCTCCAAAAATTTCCT-3'
2nd locus specific primer CE44: 5'-CCTTAAACTCATCCGTCTCTGTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11