Sequences from confirmation amplicon


Confirmation sequences for line SALK_151040 and hit At4g27460 are shown.
Line ID SALK_151040
Locus At4g27460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13733635
Primers used for PCR CE43 - LBb3, show primer details
   
Sequence Name 66-K107792-SALK_151040C-CE43
GenBank Accession LN485891 [GenBank]
Sequencing primer gene specific primer, CE43: 5'-CATGTCATTCTCCAAAAATTTCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K107792-SALK_151040C-CE43
ACAAAGTACCATGCCACGAAACAACCATCCTTCTCACCACCCACGCGTAGTTCACTCGAT
GAGCAACCGCTTGGATCATCACTGCCATTAACGAGCTCTTTGGATTACACACTATCGCCT
CCGACTTCCTTGCCATTCTTGCTGAGCTACTCATCGACCGTCCATACGACGTCGTTCTCA
CTGGAGCTTCTTCCTCCGACGAATAACCCGACGACGTCGAATAAGATGAGAGAGAATCGA
AAAGCGACATCAAACCAATCAATCCTTTATCCTCCAAACGGTTCCTAACGATCTGAACAA
GACTCTCCGGAGGATTTGCTCCGTCTATGTACGCCATCAGATCTCCAGCGGAGAGCGTCG
CCACCGCCGCAGCTGCTGTCTCGTCGCAGCAAGTTAATGTCATCGGGGATATTTCGCCGA
TCAAAGACGTAAAAGGGTCATCTCCCTCACCGTCGACCACTGCGACAGAGGTTTGGACGG
CAAGAGCGTTGGATACGGCAGAGACAACTGCGGACGCTGAGGAGTGGTAATCGACCGCAA
CAACTGTGTGTGTCATCAACACATTGCGGTGTATACAAATTGACGCTTAGACAACTTAAT
AACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGG
CAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT
GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 17-K107793-SALK_151040C-R210
GenBank Accession LN484735 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K107793-SALK_151040C-R210
GTGTCAGCGTCATTTGTATACCCGCAATGTGTTGATGACCACACAGTTGTTGCGGTCGAT
TACCACTCCTCAGCGTCCGCAGTTGTCTCTGCCGTATCCAACGCTCTTGCCGTCCAAACC
TCTGTCGCAGTGGTCGACGGTGAGGGAGATGACCCTTTTACGTCTTTGATCGGCGAAATA
TCCCCGATGACATTAACTTGCTGCGACGAGACAGCAGCTGCGGCGGTGGCGACGCTCTCC
GCTGGAGATCTGATGGCGTACATAGACGGAGCAAATCCTCCGGAGAGTCTTGTTCAGATC
GTTAGGAACCGTTTGGAGGATAAAGGATTGATTGGTTTGATGTCGCTTTTCGATTCTCTC
TCATCTTATTCGACGTCGTCGGGTTATTCGTCGGAGGAAGAAGCTCCAGTGAGAACGACG
TCGTATGGACGGTCGATGAGTAGCTCAGCAAGAATGGCAAGGAAGTCGGAGGCGATAGTG
TGTAATCCAAAGAGCTCGTTAATGGCAGTGATGATCCAAGCGGTTGCTCATCGAGTGAAC
TACGCGTGGGTGGTTGAGAAGGATGGTTGTTTCGTTGGCATGGTTACTTTTGTTGATATC
TTAAAAGTTTTTAGGAAATTTTTGGAGAATGACATG
Primers used for PCR CE44 - LBb3, show primer details
   
Sequence Name 84-K119306-SALK_151040C-CE44
GenBank Accession LN486280 [GenBank]
Sequencing primer gene specific primer, CE44: 5'-CCTTAAACTCATCCGTCTCTGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K119306-SALK_151040C-CE44
CCATCCTGCAGGTTCTTTATTCTTCCAACTCTCTCTGTTTTGAGGGTCTACATAAAAATT
CAACCTTTAGTTGTGCTTACTCTGTTTCTATGCTCTGTTTTTTTGTAATGTTCTTTAAAT
TAATGGTTTTTGTGGGGTTACTCTGGTTTGACTAATCAGCTTAATAGAAGCCATTGATCT
GATAATCAAAGGAGCACAGAATCTGATTGTCCCGATACATACAAAGCCCTACACTAAGAA
AAAGCAGCACAACGACAACGTTTCGGTCACCACCACCACCCACTCGAACGGACAACGATT
CTGCTGGATCACACAAGAAGACATCATTCAGTTTCTCCTCGGATTCATCGCCGCATTCTC
TCCTTTGCCAGCGATGTCCCTTATAAAACAAATTGACGCTTAGACAACTTAATAACACAT
TGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGC
TGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGC
CCCAGCAGGCGAAAATCCTGTTTGATGGAGGTTCCGAAATCGGCAAAA
   
Sequence Name 36-K119305-SALK_151040C-R210
GenBank Accession LN485246 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K119305-SALK_151040C-R210
CATTTGTTTTATAGGGACATCGCTGGCAAAGGAGAGAATGCGGCGATGAATCCGAGGAGA
AACTGAATGATGTCTTCTTGTGTGATCCAGCAGAATCGTTGTCCGTTCGAGTGGGTGGTG
GTGGTGACCGAAACGTTGTCGTTGTGCTGCTTTTTCTTAGTGTAGGGCTTTGTATGTATC
GGGACAATCAGATTCTGTGCTCCTTTGATTATCAGATCAATGGCTTCTATTAAGCTGATT
AGTCAAACCAGAGTAACCCCACAAAAACCATTAATTTAAAGAACATTACAAAAAAACAGA
GCATAGAAACAGAGTAAGCACAACTAAAGGTTGAATTTTTATGTAGACCCTCAAAACAGA
GAGAGTTTGGAAGAATAAAGAACCTGCAGGATGGTTGAACATGGAGGACAATGGAACGAG
TTTTGGGGAGAAGAACAGAGACGGATGAGTTTAAGGA

Last Updated on 06.12.2018 16:11