Genotyping and primer details


Genotyping and primer details for SALK_151423c - At1g10570
Line ID SALK_151423c
Locus At1g10570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3487635 of line SALK_151423c Chr1:3487635
   
Insertion allele amplicon LBb3 - 04bz
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 04bz: 5'-GGAGAAGCTTTAGCAGAATCTTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3488181
   
wt amplicon (over InsPos) 38dz - 04bz
wt amplicon start Chr1:3487066
wt amplicon end Chr1:3488181
wt amplicon size 1116 bp
Locus specific primer 04bz: 5'-GGAGAAGCTTTAGCAGAATCTTGA-3'
2nd locus specific primer 38dz: 5'-GTCCTATTCACTCACATGATGCAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11