Sequences from confirmation amplicon


Confirmation sequences for line SALK_151423c and hit At1g10570 are shown.
Line ID SALK_151423c
Locus At1g10570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3487635
Primers used for PCR 04bz - LBb3, show primer details
   
Sequence Name 92-K109033-SALK_151423C-04bz
GenBank Accession LN486346 [GenBank]
Sequencing primer gene specific primer, 04bz: 5'-GGAGAAGCTTTAGCAGAATCTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K109033-SALK_151423C-04bz
ATACTACATTATCTTCCACCATCATCAAACTCTAAATTCCTCTATCAGTTAGCTTCAGCT
ACAAATACAGAGATCACAAAAACCTTCATTTGAGTAACAGAATGATGCAATTGCAGAGTA
AGTAAAAAAAAAAAAGACAAACCCTTTTTGGCCCGTTGTTGGAACATCCTTCGCTGCTTC
TCGTACTCGAGCTCTCCAATTCTGTTTCGGATTTTCTCGCCCTTGTCTGCCAACTTTTCC
CCGAGACGAGTAAAAAGTGATCTCTGACCCTTCAAATGATCGCCGAGTTCGTTATCCCTG
AGGGATCGCACAGAGACGGGGGGTTCATAGCCGGAAAGAGTAGGTTCCCCCGGCGGAATT
TTGTCAGTAGCGATGATCTCGAGCTCGGGAACTTCCTCATCGCCCAAAGCATCGTCCCAA
TCTTTGTTCAACATTGTCTTCTTCACTCGATCTAACTCGATGGCCCTTTGTCCCTTCATC
TCTTCTTCGAATAAACTTAATAACCCATTGCGGACGTTTTTAATGTACGGGGGGGGTTTT
TCTTTTCCCCAGTGAAACGGGCAACCGCTGATTGCCCTTCCCCCCCTGGCCCTGAAAA
   
Sequence Name 76-K109032-SALK_151423C-R210
GenBank Accession LN486147 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K109032-SALK_151423C-R210
AGAGAGATGAAGAGACAAGAGCAATCGAGTTAGATCGAGTGAAGAAGACAATGTTGCACA
TAGATTGGGACGATGCTTTGGGCGATGAGGAAGTTCCCGAGCTCGAGATCATCGCTACTG
ACAAAATTCCGCCGCGTGAACCTACTCTTTCCGGCTATGAACCCGCCGTCTCTGTGCGAT
CCCTCAGGGATAACGAACTCGACGATCATCTGAAGCGTCAGAGATCACTTCTTACTCGTC
TCGGTGATAAGTTGGCAGACAAGGGCGAGAAAATCCGAAACAGAATCGGAGAGCTCGAGT
ACGAGAAGCAGCGAAGGATGTTCCAACAACGGACCAAAATGGTTTTGTCTTTTTTTTTTT
TACTTACTCGGCAATTGCATCATTCTGTTACTCAAATGAAAGTTTTTGGGACCCCGGTAT
TTGAAGCTAAAGCTAATTGTTCAGGGGAATTTAAAGTTTGATGATGTGGGGAAAAAAATG
TAGTATGGGTTTAGGAGGATCAGGGGTTGAGGGTCCCGATTGGTTCAAAATTCTGCTAAA
GCTTCC

Last Updated on 06.12.2018 16:11