Genotyping and primer details

Genotyping and primer details for SALK_151428c - At2g18590
Line ID SALK_151428c
Locus At2g18590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8070472 of line SALK_151428c Chr2:8070472
Insertion allele amplicon R204 - BI50
Locus specific primer BI50: 5'-TTTGACACATTGATATTTTTGGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8070770
wt amplicon (over InsPos) BI51 - BI50
wt amplicon start Chr2:8070026
wt amplicon end Chr2:8070770
wt amplicon size 745 bp
Locus specific primer BI50: 5'-TTTGACACATTGATATTTTTGGTT-3'
2nd locus specific primer BI51: 5'-AAATCCTCTTCATTCTCGTCTTCA-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11