Sequences from confirmation amplicon


Confirmation sequences for line SALK_151428c and hit At2g18590 are shown.
Line ID SALK_151428c
Locus At2g18590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8070472
Primers used for PCR BI50 - R204, show primer details
   
Sequence Name 47-K105819-SALK_151428C-BI50
GenBank Accession LN485428 [GenBank]
Sequencing primer gene specific primer, BI50: 5'-TTTGACACATTGATATTTTTGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K105819-SALK_151428C-BI50
TAAGAATTCCAAACTATAATGTTTCTAGATTTATGAACTTCGGTGCAAAAGTTTAAATTG
TAAGAGAAGAAAACTTGAAGTTAATGTTTTGTGGTAATTAATTAGACTGAAATTAATGGA
AAAAAACCTGGATGAAGTAGCGGCTGACTCCAGTTGCAACAGTTGATGAGACCCAAAAGA
AACTTCCGAACGCAAAAACTGTGGGACGATCATAACTGATGGCAAATAAACCGGCTAAAG
GCGAGGCAACTTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTG
GGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGC
CCTGAGAGAGTTGCAGCAACGGGTCCACG
   
Sequence Name 63-K105820-SALK_151428C-R210
GenBank Accession LN485809 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K105820-SALK_151428C-R210
GTCAGTTGCCTCGCCTTTAGCCGGTTTATTTGCCATCAGTTATGATCGTCCCACAGTTTT
TGCGTTCGGAAGTTTCTTTTGGGTCTCATCAACTGTTGCAACTGGAGTCAGCCGCTACTT
CATCCAGGTTTTTTTCCATTAATTTCAGTCTAATTAATTACCACAAAACATTAACTTCAA
GTTTTCTTCTCTTACAATTTAAACTTTTGCACCGAAGTTCATAAATCTTAGAAACATTAT
ATGTTTGGTAATTTTCTTTTACAAATATGACAAAAGCAATAACCAAAAATATCAATGTGT
CAA

Last Updated on 06.12.2018 16:11