Genotyping and primer details


Genotyping and primer details for SALK_151487c - At3g56880
Line ID SALK_151487c
Locus At3g56880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21060077 of line SALK_151487c Chr3:21060077
   
Insertion allele amplicon LBb3 - 27mh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 27mh: 5'-AATTGGAATGTTGAAAATAGCCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21059425
   
wt amplicon (over InsPos) 11nl - 27mh
wt amplicon start Chr3:21059425
wt amplicon end Chr3:21060174
wt amplicon size 750 bp
Locus specific primer 27mh: 5'-AATTGGAATGTTGAAAATAGCCTG-3'
2nd locus specific primer 11nl: 5'-GATCTGTGAAGAGCTTTAGCGAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11