Sequences from confirmation amplicon


Confirmation sequences for line SALK_151487c and hit At3g56880 are shown.
Line ID SALK_151487c
Locus At3g56880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21060077
Primers used for PCR 27mh - LBb3, show primer details
   
Sequence Name 20-K114334-SALK_151487C-27mh
GenBank Accession LN484855 [GenBank]
Sequencing primer gene specific primer, 27mh: 5'-AATTGGAATGTTGAAAATAGCCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K114334-SALK_151487C-27mh
AGTGAGCATGCTATATACATATTGTTTCTTTGTTCACTCTATTTTCCACTAATTTTCTTT
TAAAAAGATTAACAATTAAGTACTTAAATGTGTCAAATCAGCTATATAACCTTGAGATAT
TTAGGAGCAAAAATAATTTATATTTTTACAAGACCAATTCTCAATCCATCTTTTTTTTAT
AATAAAAATGTTCAAAGAAGAGATTAGCGACGACATAATCCATAAATTAATACAGAGATT
AGATAGAGAGTCAAGAAAAATGGGTTTAATAAATAAGACGAAAAAAACAGAGCTGAGTCA
GAGTTCCGTACAATTCTGCTGACGCGTGTCTTCTCCTTTCTCTCTCTTCTCCACGCGGTT
TCTCATTCTCTCTCTCTCTCTCTTCGCCGTCCTATTTATATTATCTCTCCTCAGCTTTCA
TTTCTCCAATTCATTCATTATCACATTCCTTTACTCACTCTCTCTCTCTCTCTAAAAACA
GAGCTCAACAATAATGATCCACTAAAAAAGATTCTCTTTTCTCTTCTTCTCCGGTGATTT
ACTTACTTAACAATCAGGGCGTCGTCAGAGGGATTAGCAAGCGTTGATCCCATTGACGCT
NAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCA
CCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCGGGCCCTGAGAGAGTTGCAGCA
AGCGGTCCACGCTGGTGTGCCCCAGCAGGCAAAAATCCTGTTTGATGGTGG
   
Sequence Name 10-K114333-SALK_151487C-R210
GenBank Accession LN484556 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K114333-SALK_151487C-R210
GATCACGCTTGCTATCCCTCCGACGACGCCATGATTGTTAAGTAAGTAAATCACCGGAGA
AGAAGAGAAAAGAGAATCTTTTTTAGTGGATCATTATTGTTGAGCTCTGTTTTTAGAGAG
AGAGAGAGAGTGAGTAAAGGAATGTGATAATGAATGAATTGGAGAAATGAAAGCTGAGGA
GAGATAATATAAATAGGACGGCGAAGAGAGAGAGAGAGAGAATGAGAAACCGCGTGGAGA
AGAGAGAGAAAGGAGAAGACACGCGTCAGCAGAATTGTACGGAACTCTGACTCAGCTCTG
TTTTTTTCGTCTTATTTATTAAACCCATTTTTCTTGACTCTCTATCTAATCTCTGTATTA
ATTTATGGATTATGTCGTCGCTAATCTCTTCTTTGAACATTTTTATTATAAAAAAAAGAT
GGATTGAGAATTGGTCTTGTAAAAATATAAATTATTTTTGCTCCTAAATATCTCAGGGTT
ATATAGCTGATTTGACACATTTAAGTACTTAATTGTTAATCTTTTTAAAAGAAAATTAGT
GGAAAATAGAGTTGAACAAAGAAACAATATTGTATATNGCATTGCTCACTTCTACATGTA
GAGTGTTCGACACAGTCCAAATGTAGCCGGGCTATTTTCAC

Last Updated on 06.12.2018 16:11