Genotyping and primer details


Genotyping and primer details for SALK_151601c - At1g53290
Line ID SALK_151601c
Locus At1g53290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19872246 of line SALK_151601c Chr1:19872246
   
Insertion allele amplicon LBb3 - 54fm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 54fm: 5'-TACATTCTCTTGCCATTGTTCTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19871723
   
wt amplicon (over InsPos) 11go - 54fm
wt amplicon start Chr1:19871723
wt amplicon end Chr1:19872629
wt amplicon size 907 bp
Locus specific primer 54fm: 5'-TACATTCTCTTGCCATTGTTCTTG-3'
2nd locus specific primer 11go: 5'-AGATCTGCGTTTCAACTTTTTCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11