Sequences from confirmation amplicon


Confirmation sequences for line SALK_151601c and hit At1g53290 are shown.
Line ID SALK_151601c
Locus At1g53290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19872246
Primers used for PCR 54fm - LBb3, show primer details
   
Sequence Name 87-K111509-SALK_151601C-54fm
GenBank Accession LN486306 [GenBank]
Sequencing primer gene specific primer, 54fm: 5'-TACATTCTCTTGCCATTGTTCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K111509-SALK_151601C-54fm
ATCTGTGGATCATTGCATCACTTAGAATTTGTTTGATCTGGATTTGAATTAGCAAATCTG
AGAGTTTTATGTGGATCTTACTTAATAGATTGAAGTGAAGGCAATGAGGTTTGATTTTGT
ATTGATCTTGATTCTTAGGTTGAAATGAATCTAATTAGGTGCTTCTTAGTGAACTTTAGT
TTCCAAAGTCTCACCAGATTCAAGAAAATTGATTCTTGGATGCTTTGACGATAGAGATGG
ATCTGCTTGTGTTTACTTTGTCACTGAGTAACACTGCTCACTGCTGTTTGCAGTTTGGAA
GAATCTACGGGGTTAGCCATCAGGTTTATGATAGGAAAAACGAAAAGTGAGGAGAAAATG
GCTCAGCTCAGAAGAGAAATCGCAGAGTATGATGACTTCGTACTGCTAGATATCGAAGAG
GAGTACAGTAAGCTTCCTTACAAAACGTTAGTGAGAGTGATTTGGTTCAGGATATATTGT
GGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTAC
TGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTG
GCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTG
TTTGATGGTGGTTC
   
Sequence Name 73-K111508-SALK_151601C-R210
GenBank Accession LN486075 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K111508-SALK_151601C-R210
CATTTGTTACCCACAATATATCCTGAACCAATCACTCTCACTAACGTTTTGTAAGGAAGC
TTACTGTACTCCTCTTCGATATCTAGCAGTACGAAGTCATCATACTCTGCGATTTCTCTT
CTGAGCTGAGCCATTTTCTCCTCACTTTTCGTTTTTCCTATCATAAACCTGATGGCTAAC
CCCGTAGATTCTTCCAAACTGCAAACAGCAGTGAGCAGTGTTACTCAGTGACAAAGTAAA
CACAAGCAGATCCATCTCTATCGTCAAAGCATCCAAGAATCAATTTTCTTGAATCTGGTG
AGACTTTGGAAACTAAAGTTCACTAAGAAGCACCTAATTAGATTCATTTCAACCTAAGAA
TCAAGATCAATACAAAATCAAACCTCATTGCCTTCACTTCAATCTATTAAGTAAGATCCA
CATAAAACTCTCAGATTTGCTAATTCAAATCCAGATCAAACAAATTCTAAGTGATGCAAT
GATTCCACAGATATCCTGAAGCTAATTCAAACAGATGCTCACAAGAACAATGGCAAGAGA
ATGT

Last Updated on 06.12.2018 16:11