Genotyping and primer details


Genotyping and primer details for SALK_151687c - At1g49910
Line ID SALK_151687c
Locus At1g49910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:18479795 of line SALK_151687c Chr1:18479795
   
Insertion allele amplicon LBb3 - 20db
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 20db: 5'-CTTCCCTCTTTCTCTCTGAGCATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18478975
   
wt amplicon (over InsPos) 40dz - 20db
wt amplicon start Chr1:18478975
wt amplicon end Chr1:18479828
wt amplicon size 854 bp
Locus specific primer 20db: 5'-CTTCCCTCTTTCTCTCTGAGCATA-3'
2nd locus specific primer 40dz: 5'-AAACGATTTCCAACAAGAGAAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11