Genotyping and primer details


Genotyping and primer details for SALK_151769c - At1g19370
Line ID SALK_151769c
Locus At1g19370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6694744 of line SALK_151769c Chr1:6694744
   
Insertion allele amplicon LBb3 - 50sf
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 50sf: 5'-TCTCTCTTTCTCTTCTCCGATGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6694419
   
wt amplicon (over InsPos) 09tl - 50sf
wt amplicon start Chr1:6694419
wt amplicon end Chr1:6695366
wt amplicon size 948 bp
Locus specific primer 50sf: 5'-TCTCTCTTTCTCTTCTCCGATGTC-3'
2nd locus specific primer 09tl: 5'-GGGATCATAGTGGACTTTGTCAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11