Genotyping and primer details


Genotyping and primer details for Salk_020809 - At1g28090
Line ID Salk_020809
Locus At1g28090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:9795636 of line Salk_020809 Chr1:9795636
   
Insertion allele amplicon LBb3 - 06bh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 06bh: 5'-CCTTTTTGGCTTATCCATTAACAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:9795042
   
wt amplicon (over InsPos) 16cr - 06bh
wt amplicon start Chr1:9795042
wt amplicon end Chr1:9795816
wt amplicon size 775 bp
Locus specific primer 06bh: 5'-CCTTTTTGGCTTATCCATTAACAA-3'
2nd locus specific primer 16cr: 5'-TTACGAGAGTCGGAGAAGAAGAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11