Sequences from confirmation amplicon


Confirmation sequences for line 258C09 and hit At1g02405 are shown.
Line ID 258C09
Locus At1g02405 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:490273
Primers used for PCR 13lg - 8474, show primer details
   
Sequence Name 47-K114086-0022-258-C09C-13lg
GenBank Accession LN506264 [GenBank]
Sequencing primer gene specific primer, 13lg: 5'-CACTGCAGTTGTACTCTCGATCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K114086-0022-258-C09C-13lg
TAACTCTTATCAAATCGGCTATACTTATCTAGGTATGCTTGATTTCATTTGAAGGTTTTA
TAAAAGGTTGCAGGGGACAAGTCCAAATAATATGAACCACATCTGACCCTAACTATAAAA
ATTAATCCACAATCGATTATAATTATTAAAAAAAACTGTATCTAAAGCATACGCCAATCC
GTTAAAAGTAAACAAAATCTTTATGCAAACCTTATAGAAGTTCGAAAACAACAACATAAA
TAATTTCTGATCATAATCAAAGAATCAGAAAAACCATAACTCCAAACACGAACAGCTTCA
TCACCGAAAAGCTTTCTACTGCCACAGCCGCTCCATAGTAATTTTCAATAGGGTACAAGT
CTCCCGGCGGATATGTGAATACGTAATTAGGTGGAGGTGGAGGTGGCGGTGGCGGCAGAG
GCGATGGTGGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 71-K114085-0022-258-C09C-8409
GenBank Accession LN512597 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K114085-0022-258-C09C-8409
GCCTTTACATTGAATATTCCTGTCCACCATCGCCTCTGCCGCCACCGCCACCTCCACCTC
CACCTAATTACGTATTCACATATCCGCCGGGAGACTTGTACCCTATTGAAAATTACTATG
GAGCGGCTGTGGCAGTAGAAAGCTTTTCGGTGATGAAGCTGTTCGTGTTTGGAGTTATGG
TTTTTCTGATTCTTTGATTATGATCAGAAATTATTTATGTTGTTGTTTTCGAACTTCTAT
AAGGTTTGCATAAAGATTTTGTTTACTTTTAACGGATTGGCGTATGCTTTAGATACAGTT
TTTTTTAATAATTATAATCGATTGTGGATTAATTTTTATAGTTAGGGTCAGATGTGGTTC
ATATTATTTGGACTTGTCCCCTGCAACCTTTTATAAAACCTTCAAATGAAATCAAGCATA
CCTAGATAAGTAATAGCCGATTTTGTATAAAGAGGTTTTAATTCGTGTAATTATGATCGA
GAGTACAACTGCAGTG

Last Updated on 06.12.2018 16:11