Sequences from confirmation amplicon


Confirmation sequences for line 205G08 and hit At1g03060 are shown.
Line ID 205G08
Locus At1g03060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:725075
Primers used for PCR MZ78 - 8474, show primer details
   
Sequence Name 07-K026796-022-205-G08C-8409
GenBank Accession LN489113 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K026796-022-205-G08C-8409
GGTAGTGTTGTTCATATCATGAAAGCATTAGCAAGCAATCNCTTCTGGCTGCTCAGAGTT
TGATTGAAGATGACTCTCTTGAGTCGCTTTTCAATATGGTTGCCAATGGCTCCATTACAG
TTTTTTCTCAGTATAAAGAAGGTCTAGTTCCCCTCCATAACATACAACTTCACAGACATG
CAATGCAGGTAATTTTTGCGCATCTATTTTAGAGCATCATGCTATGAAATGGAAAAATTC
CGTGAACGGCTTTCTAGATGGACAAATTAAAACTGGATTGAATAATCTGATGCTTATACT
TTCATTTGGCACCCTTTCGTGTCTCTTCCAGATCCTTGGACTTCTACTAGTCAATGACAA
TGGGAGCACAGCAAGATACATACGCAAGCACCATCTGGTACGCCAGTGAATTATGCAGAC
TGCCTGAGTGTAGTATATTGAGTCTCATGCATACTTTTAAGCTGACCGTGGGTATAGTTT
ATTCTTTTTGCTAATGTTGAAACTATG
   
Sequence Name 08-K026796-022-205-G08C-MZ78
GenBank Accession LN489576 [GenBank]
Sequencing primer gene specific primer, MZ78: 5'-CACGGAAATCTCACATAGTTTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K026796-022-205-G08C-MZ78
GCATTAGCAAAAAGAATAAACTATACCCACGGTCAGCTTAAAAGTATGCATGAGACTCAA
TATACTACACTCAGGCAGTCTGCATAATTCACTGGCGTACCAGATGGTGCTTGCGTATGT
ATCTTGCTGTGCTCCCATTGTCATTGACTAGTAGAAGTCCAAGGATCTGGAAGAGACACG
AAAGGGTGCCAAATGAAAGTATAAGCATCAGATTATTCAATCCAGTTTTAATTTGTCCAT
CTAGAAAGCCGTTCACGGAATTTTTCCATTTCATAGCATGATGCTCTAAAATAGATGCGC
AAAAATTACCTGCATTGCATGTCTGTGAAGTTGTATGTTATGGAGGGGAACTAGACCTTC
TTTATACTGAGAAAAAACTGTAATGGAGCCATTGGCAACCATATTGAAAAGCGACTCAAG
AGAGTCATCTTCAATCAAACTCTGAGCAGCAGAAGGATTGCTTGCTAATGCTTTCATGAT
ATGAACAACACTACCCTCAATGAGTAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAAT
GAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAAT
AAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11