Sequences from confirmation amplicon


Confirmation sequences for line 220D11 and hit At1g05717 are shown.
Line ID 220D11
Locus At1g05717 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr:
Primers used for PCR MK02 - 8474, show primer details
   
Sequence Name 28-K021339-022-220-D11C-8409
GenBank Accession LN498661 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K021339-022-220-D11C-8409
GATTTCCCGGACATGAAGCCATTTACAATTGAATATATTCACTAAACTCATAAAAGTTAT
CAAACTCATAAAAGTTAGCGTCTTTGATGCTTAAACAGCATATGAGTATCTCGTAATCTC
ATCACCGTTTATCCAAAGATTTTGATGAAAAAAGAAGAGCAAACTTAAGAAATCTCCAAA
ACGATATACAACGAAACGAGGCTAGAGAAGGAATATAGTCAAAAATTCTAATTTTAGTTC
CTATTAACTCGCCTAATTATAAGCAGTTTTCTTCATCCTCCTTCCGTATATATTAACAAA
ATTATTTTCAAACAAGGATTTTTTGGATTGAAGTTTTGTAGATTACTGATCAGCATTATT
ATATCAACTTACGATTTGGATGAATCAAAATCTCTTCCTGCTTCGCCAATATGGGTCGGG
TGAAATTAGAAATCAAGAGAATAGAGAACACAACGAATC
   
Sequence Name 29-K021339-022-220-D11C-MK02
GenBank Accession LN499105 [GenBank]
Sequencing primer gene specific primer, MK02: 5'-GTAACTTGTCGATTCGTTGTGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K021339-022-220-D11C-MK02
CTCTTGATTTCTAATTTCACCCGACCCATATTGGCGAAGCAGGAAGAGATTTTGATTCAT
CCAAATCGTAAGTTGATATAATAATGCTGATCAGTAATCTACAAAACTTCAATCCAAAAA
ATCCTTGTTTGAAAATAATTTTGTTAATATATACGGAAGGAGGATGAAGAAAACTGCTTA
TAATTAGGCGAGTTAATAGGAACTAAAATTAGAATTTTTGACTATATTCCTTCTCTAGCC
TCGTTTCGTTGTATATCGTTTTGGAGATTTCTTAAGTTTGCTCTTCTTTTTTCATCAAAA
TCTTTGGATAAACGGTGATGAGATTACGAGATACTCATATGCTGTTTAAGCATCAAAGAC
GCTAACTTTTATGAGTTTGATAACTTTTATGAGTTTAGTGAATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGA
AAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTAC
Primers used for PCR MK03 - 8474, show primer details
   
Sequence Name 01-K021340-022-220-D11C-8409
GenBank Accession LN486394 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K021340-022-220-D11C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTATGAACTATCTTCAC
TAAAAATGTCCCATTAACACAATTTCAGTTAATGTCTCGACTTTTGTTTGCCTTTGAATA
TATATCAAACATTGAGGGATGTAACATTCAACCACCAAATTTCCAAATAATCAGAGCCAA
CGATTAAGTCACAATTACTGAGGACTAAATTTCTGATTTAACACTTGAGACAAAAGTGGG
AACATGAGAGTACAACAACAAAAGAGAAACAGAGTTGGATCACAAATCATCGAGGAAATG
TCTTAAATCACCTGAAACACACATAAATAGACAACATCAATAGATAATGCAGTAACAGAT
CTCTTTAGCTAGTTTCTTGAGATGAAACAAAAAAACACAACAATGGTTTCATCAGATCAC
ACACAATCTGCAGATCCATTGTCCCAATTCATAAACTCAGATGCAAGTAGCAGTACTAAT
GAGAGTCGAATGATCTCTGTTACCTATGAAGAAGTAGTGAATAGACCAGAATCAATGATG
   
Sequence Name 02-K021340-022-220-D11C-MK03
GenBank Accession LN486849 [GenBank]
Sequencing primer gene specific primer, MK03: 5'-TCGACGATACTCATCATTGATTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K021340-022-220-D11C-MK03
ATTCACTACATTCTTCATAGGTAACAGAGATCATTCGACTCTCATTAGTACTGCTACTTG
CATCTGAGTTTATGAATTGGGACAATGGATCTGCAGATTGTGTGTGATCTGATGAAACCA
TTGTTGTGTTTTTTTGTTTCATCTCAAGAAACTAGCTAAAGAGATCTGTTACTGCATTAT
CTATTGATGTTGTCTATTTATGTGTGTTTCAGGTGATTTAAGACATTTCCTCGATGATTT
GTGATCCAACTCTGTTTCTCTTTTGTTGTTGTACTCTCATGTTCCCACTTTTGTCTCAAG
TGTTAAATCAGAAATTTAGTCCTCAGTAATTGTGACTTAATCGTTGGCTCTGATTATTTG
GAAATTTGGTGGTTGAATGTTACATCCCTCAATGTTTGATATATATTCAAAGGCAAACAA
AAGTCGAGACATTAACTGAAATTGTGTTAATGGGACATTTTTAGTGAAGATAGTTCATAC
AGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
GATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTAT
NTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11